For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp. The fluorescence attachment have a rail for maximum 4 filter cubes. The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome. Other FluoLED wavelengths are also available
Oxion Fluorescence Microscope
Technical Specification
Binocular Oxion microscope for fluorescence with Plan PL 4/10/S40/S100x oil IOS objectives and FluoLED 455 nm attachment
HWF 10x/22 mm eyepieces
Binocular head, 30° inclined tubes. Tubes can be positioned in upper or lower positions Interpupillary distance 50-75 mm
Diopter adjustment on both eyepieces
Reversed quintuple revolving nosepiece on ball-bearings
Plan PL 4x, 10x, S40x, S100x oil immersion infinity corrected IOS objectives
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput 150 x 140 mm stage with 76 x 50 mm mechanical stage
Coaxial coarse and fine adjustments with 200 graduations. Precision 1 μm, 0.2 mm per rotation. Total travel approximately 28 mm
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm and filter holder Condenser with slot for optional slider with darkfield or phase contrast
Diascopic 3 W NeoLEDTM Köhler illumination with adjustable intensity with internal power supply 100-240 V (CE)
Reflected 455 nm LED epi-illumination attachment with a single cube with filters for Auramine-O fluorochrome EX 425-475 DM 485 EM 495 LP. External power supply Supplied with power cord, dust cover and 5 ml oil immersion.
